{"id":13339,"date":"2024-10-02T11:51:35","date_gmt":"2024-10-02T04:51:35","guid":{"rendered":"https:\/\/plantscience.sc.mahidol.ac.th\/?p=13339"},"modified":"2024-10-02T11:52:31","modified_gmt":"2024-10-02T04:52:31","slug":"genome-wide-identification-and-data-mining-reveals-major-latex-protein-mlp-from-the-pr-10-protein-family-played-defense-related-roles-against-phytopathogenic-challenges-in-cassava-manihot-esculenta-2","status":"publish","type":"post","link":"https:\/\/plantscience.sc.mahidol.ac.th\/?p=13339&lang=en","title":{"rendered":"Genome-wide identification and data mining reveals major-latex protein (MLP) from the PR-10 protein family played defense-related roles against phytopathogenic challenges in cassava (<i>Manihot esculenta<\/i> Crantz)"},"content":{"rendered":"<p><style>\n  @import url('https:\/\/fonts.googleapis.com\/css2?family=K2D:wght@300&display=swap');<br \/>\n<\/style>\n<\/p>\n<h5>Genetica. 2024 Aug 31:1-4.<br \/>\n<a href=\"https:\/\/doi.org\/10.1007\/s10709-024-00211-6\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.1007\/s10709-024-00211-6<\/a><\/h5>\n<p style=\"text-indent: 3em; text-align: justify; font-family: 'K2D', sans-serif; font-size: 1.0em;\">\nAlthough previously identified in some studies, the pathogenesis-related 10 (PR-10) protein has not been well studied and remains underexplored in many plant species. This research uses data mining techniques to study PR-10 proteins in cassava, a key global crop. The study focused on 53 PR-10 proteins found in cassava using in silico methods. These proteins are divided into two main groups: 34 major latex proteins (MLPs) and 13 major allergen proteins, called Pru ar 1, based on their evolutionary relationships. A genome comparison with the rubber tree suggests a possible evolutionary link between PR-10 genes in these two Euphorbiaceae species, particularly on chromosome 15. Notably, MLP423 and other MLP proteins were found in several published cassava transcriptome datasets, showing responses to pathogens like anthracnose fungus, viruses, and bacterial blight. Ligand prediction and molecular docking of three MLP423 proteins showed possible interactions with cytokinin and abscisic acid hormones. Their expression levels and predicted binding strengths are discussed, emphasizing their role in cassava\u2019s defense against major diseases.<\/p>\n<p style=\"text-indent: 3em; text-align: justify; font-family: 'K2D', sans-serif; font-size: 1.0em;\">\n\u0e07\u0e32\u0e19\u0e27\u0e34\u0e08\u0e31\u0e22\u0e19\u0e35\u0e49\u0e27\u0e34\u0e40\u0e04\u0e23\u0e32\u0e30\u0e2b\u0e4c\u0e02\u0e49\u0e2d\u0e21\u0e39\u0e25\u0e02\u0e2d\u0e07\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19 PR-10 \u0e43\u0e19\u0e21\u0e31\u0e19\u0e2a\u0e33\u0e1b\u0e30\u0e2b\u0e25\u0e31\u0e07\u0e0b\u0e36\u0e48\u0e07\u0e40\u0e1b\u0e47\u0e19\u0e1e\u0e37\u0e0a\u0e40\u0e28\u0e23\u0e29\u0e10\u0e01\u0e34\u0e08\u0e2a\u0e33\u0e04\u0e31\u0e0d\u0e02\u0e2d\u0e07\u0e44\u0e17\u0e22 \u0e42\u0e14\u0e22\u0e17\u0e33\u0e01\u0e32\u0e23\u0e23\u0e30\u0e1a\u0e38\u0e0a\u0e19\u0e34\u0e14\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e43\u0e19\u0e01\u0e25\u0e38\u0e48\u0e21 PR-10 \u0e14\u0e49\u0e27\u0e22\u0e27\u0e34\u0e18\u0e35\u0e01\u0e32\u0e23\u0e17\u0e32\u0e07\u0e0a\u0e35\u0e27\u0e2a\u0e32\u0e23\u0e2a\u0e19\u0e40\u0e17\u0e28 \u0e1e\u0e1a\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19 PR-10 \u0e17\u0e31\u0e49\u0e07\u0e2b\u0e21\u0e14 53 \u0e0a\u0e19\u0e34\u0e14\u0e17\u0e35\u0e48\u0e2a\u0e32\u0e21\u0e32\u0e23\u0e16\u0e41\u0e1a\u0e48\u0e07\u0e2d\u0e2d\u0e01\u0e40\u0e1b\u0e47\u0e19\u0e2a\u0e2d\u0e07\u0e01\u0e25\u0e38\u0e48\u0e21 \u0e44\u0e14\u0e49\u0e41\u0e01\u0e48 \u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19 major latex proteins (MLPs) \u0e08\u0e33\u0e19\u0e27\u0e19 34 \u0e0a\u0e19\u0e34\u0e14 \u0e41\u0e25\u0e30\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e17\u0e35\u0e48\u0e01\u0e48\u0e2d\u0e43\u0e2b\u0e49\u0e40\u0e01\u0e34\u0e14\u0e2d\u0e32\u0e01\u0e32\u0e23\u0e41\u0e1e\u0e49\u0e43\u0e19\u0e01\u0e25\u0e38\u0e48\u0e21 Pru ar 1 \u0e08\u0e33\u0e19\u0e27\u0e19 13 \u0e0a\u0e19\u0e34\u0e14 \u0e41\u0e25\u0e30\u0e22\u0e31\u0e07\u0e1e\u0e1a\u0e2d\u0e35\u0e01\u0e27\u0e48\u0e32\u0e22\u0e35\u0e19\u0e02\u0e2d\u0e07\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e14\u0e31\u0e07\u0e01\u0e25\u0e48\u0e32\u0e27\u0e21\u0e35\u0e04\u0e27\u0e32\u0e21\u0e2a\u0e2d\u0e14\u0e04\u0e25\u0e49\u0e2d\u0e07\u0e01\u0e31\u0e1a\u0e22\u0e35\u0e19\u0e02\u0e2d\u0e07\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e40\u0e14\u0e35\u0e22\u0e27\u0e01\u0e31\u0e19\u0e1a\u0e19\u0e08\u0e35\u0e42\u0e19\u0e21\u0e02\u0e2d\u0e07\u0e22\u0e32\u0e07\u0e1e\u0e32\u0e23\u0e32 \u0e41\u0e2a\u0e14\u0e07\u0e43\u0e2b\u0e49\u0e40\u0e2b\u0e47\u0e19\u0e16\u0e36\u0e07\u0e04\u0e27\u0e32\u0e21\u0e40\u0e1b\u0e47\u0e19\u0e44\u0e1b\u0e44\u0e14\u0e49\u0e43\u0e19\u0e04\u0e27\u0e32\u0e21\u0e2a\u0e31\u0e21\u0e1e\u0e31\u0e19\u0e18\u0e4c\u0e17\u0e32\u0e07\u0e27\u0e34\u0e27\u0e31\u0e12\u0e19\u0e32\u0e01\u0e32\u0e23\u0e02\u0e2d\u0e07\u0e22\u0e35\u0e19 PR-10 \u0e23\u0e30\u0e2b\u0e27\u0e48\u0e32\u0e07\u0e1e\u0e37\u0e0a\u0e43\u0e19\u0e2a\u0e01\u0e38\u0e25 Euphorbiaceae \u0e17\u0e31\u0e49\u0e07\u0e2a\u0e2d\u0e07\u0e0a\u0e19\u0e34\u0e14 \u0e42\u0e14\u0e22\u0e40\u0e09\u0e1e\u0e32\u0e30\u0e1a\u0e19\u0e42\u0e04\u0e23\u0e42\u0e21\u0e42\u0e0b\u0e21\u0e17\u0e35\u0e48 15 \u0e19\u0e2d\u0e01\u0e08\u0e32\u0e01\u0e19\u0e35\u0e49\u0e43\u0e19\u0e10\u0e32\u0e19\u0e02\u0e49\u0e2d\u0e21\u0e39\u0e25\u0e17\u0e23\u0e32\u0e19\u0e2a\u0e04\u0e23\u0e34\u0e1b\u0e42\u0e15\u0e21\u0e02\u0e2d\u0e07\u0e21\u0e31\u0e19\u0e2a\u0e33\u0e1b\u0e30\u0e2b\u0e25\u0e31\u0e07\u0e41\u0e2a\u0e14\u0e07\u0e43\u0e2b\u0e49\u0e40\u0e2b\u0e47\u0e19\u0e27\u0e48\u0e32 \u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19 MLP423 \u0e41\u0e25\u0e30\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19 MLP \u0e1a\u0e32\u0e07\u0e0a\u0e19\u0e34\u0e14 \u0e21\u0e35\u0e01\u0e32\u0e23\u0e15\u0e2d\u0e1a\u0e2a\u0e19\u0e2d\u0e07\u0e15\u0e48\u0e2d\u0e40\u0e0a\u0e37\u0e49\u0e2d\u0e01\u0e48\u0e2d\u0e42\u0e23\u0e04\u0e1e\u0e37\u0e0a \u0e23\u0e27\u0e21\u0e16\u0e36\u0e07\u0e40\u0e0a\u0e37\u0e49\u0e2d\u0e23\u0e32\u0e41\u0e2d\u0e19\u0e41\u0e17\u0e23\u0e04\u0e42\u0e19\u0e2a \u0e44\u0e27\u0e23\u0e31\u0e2a \u0e41\u0e25\u0e30\u0e41\u0e1a\u0e04\u0e17\u0e35\u0e40\u0e23\u0e35\u0e22 \u0e01\u0e32\u0e23\u0e17\u0e33\u0e19\u0e32\u0e22\u0e01\u0e32\u0e23\u0e08\u0e31\u0e1a\u0e15\u0e31\u0e27\u0e02\u0e2d\u0e07\u0e42\u0e21\u0e40\u0e25\u0e01\u0e38\u0e25\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19 MLP423 \u0e01\u0e31\u0e1a\u0e25\u0e34\u0e41\u0e01\u0e19\u0e14\u0e4c \u0e40\u0e1c\u0e22\u0e43\u0e2b\u0e49\u0e40\u0e2b\u0e47\u0e19\u0e04\u0e27\u0e32\u0e21\u0e40\u0e1b\u0e47\u0e19\u0e44\u0e1b\u0e44\u0e14\u0e49\u0e43\u0e19\u0e01\u0e32\u0e23\u0e21\u0e35\u0e1b\u0e0f\u0e34\u0e2a\u0e31\u0e21\u0e1e\u0e31\u0e19\u0e18\u0e4c\u0e01\u0e31\u0e1a\u0e2e\u0e2d\u0e23\u0e4c\u0e42\u0e21\u0e19\u0e1e\u0e37\u0e0a \u0e44\u0e0b\u0e42\u0e15\u0e44\u0e04\u0e19\u0e34\u0e19 \u0e41\u0e25\u0e30\u0e01\u0e23\u0e14\u0e41\u0e2d\u0e1a\u0e44\u0e0b\u0e0b\u0e34\u0e01 \u0e01\u0e32\u0e23\u0e41\u0e2a\u0e14\u0e07\u0e2d\u0e2d\u0e01\u0e02\u0e2d\u0e07\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e01\u0e25\u0e38\u0e48\u0e21\u0e19\u0e35\u0e49\u0e41\u0e25\u0e30\u0e04\u0e27\u0e32\u0e21\u0e2a\u0e32\u0e21\u0e32\u0e23\u0e16\u0e43\u0e19\u0e01\u0e32\u0e23\u0e08\u0e31\u0e1a\u0e01\u0e31\u0e1a\u0e2e\u0e2d\u0e23\u0e4c\u0e42\u0e21\u0e19\u0e1e\u0e37\u0e0a\u0e14\u0e31\u0e07\u0e01\u0e25\u0e48\u0e32\u0e27 \u0e41\u0e2a\u0e14\u0e07\u0e16\u0e36\u0e07\u0e1a\u0e17\u0e1a\u0e32\u0e17\u0e02\u0e2d\u0e07 PR-10 \u0e43\u0e19\u0e15\u0e2d\u0e1a\u0e2a\u0e19\u0e2d\u0e07\u0e41\u0e25\u0e30\u0e1b\u0e49\u0e2d\u0e07\u0e01\u0e31\u0e19\u0e01\u0e32\u0e23\u0e40\u0e01\u0e34\u0e14\u0e42\u0e23\u0e04\u0e2a\u0e33\u0e04\u0e31\u0e0d\u0e43\u0e19\u0e21\u0e31\u0e19\u0e2a\u0e33\u0e1b\u0e30\u0e2b\u0e25\u0e31\u0e07<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Genetica. 2024 Aug 31:1-4. https:\/\/doi.org\/10.1007\/s10709-024-00211-6 Although previously identified in some studies, the pathogenesis-related 10 (PR-10) protein has not been well studied and remains underexplored in many plant species. This research uses data mining techniques to study PR-10 proteins in cassava, a key global crop. The study focused on 53 PR-10 proteins found in cassava using [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":13338,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[70,79],"tags":[],"class_list":["post-13339","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publication","category-uncategorized-en"],"_links":{"self":[{"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/posts\/13339","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13339"}],"version-history":[{"count":2,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/posts\/13339\/revisions"}],"predecessor-version":[{"id":13341,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/posts\/13339\/revisions\/13341"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/media\/13338"}],"wp:attachment":[{"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13339"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13339"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}