{"id":13343,"date":"2024-10-02T11:59:27","date_gmt":"2024-10-02T04:59:27","guid":{"rendered":"https:\/\/plantscience.sc.mahidol.ac.th\/?p=13343"},"modified":"2024-10-02T11:59:27","modified_gmt":"2024-10-02T04:59:27","slug":"in-silico-analysis-of-secreted-effectorome-of-the-rubber-tree-pathogen-rigidoporus-microporus-highlights-its-potential-virulence-proteins-2","status":"publish","type":"post","link":"https:\/\/plantscience.sc.mahidol.ac.th\/?p=13343","title":{"rendered":"<i>In silico<\/i> analysis of secreted effectorome of the rubber tree pathogen <i>Rigidoporus microporus<\/i> highlights its potential virulence proteins"},"content":{"rendered":"<p><style>\n  @import url('https:\/\/fonts.googleapis.com\/css2?family=K2D:wght@300&display=swap');<br \/>\n<\/style>\n<\/p>\n<h5>Frontiers in Microbiology 15 (2024): 1439454<br \/>\n<a href=\"https:\/\/doi.org\/10.3389\/fmicb.2024.1439454\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.3389\/fmicb.2024.1439454<\/a><\/h5>\n<p style=\"text-indent: 3em; text-align: justify; font-family: 'K2D', sans-serif; font-size: 1.0em;\">\n<i>Rigidoporus microporus<\/i>, the fungus that causes white root rot disease in rubber trees, is a major threat to natural rubber production worldwide. Understanding how this fungus causes disease is important for developing better ways to manage it. The fungus releases effector proteins, which help it weaken the rubber tree&#8217;s defenses and cause infection. In this study, we used computer analyses to identify 357 possible effector proteins from the <i>R. microporus<\/i> genome. This information were combined with previous RNA-seq data to study how these proteins respond to rubber tree latex. Many of the identified effectors belong to protein families linked to the fungus&#8217;s ability to cause disease, especially hydrophobin and glycoside hydrolase (GH) proteins. The role of these secreted effectors in helping the fungus infect rubber trees was discussed, particularly how they react to rubber tree latex. The structures of some unknown, highly active effectors were predicted and found to resemble proteins like aminopeptidase, ubiquitin ligase, spherulin, and thaumatin. This study helps explain how <i>R. microporus<\/i> infects rubber trees and suggests new targets for controlling white root rot disease in rubber plantations.<\/p>\n<p style=\"text-indent: 3em; text-align: justify; font-family: 'K2D', sans-serif; font-size: 1.0em;\">\n<i>Rigidoporus microporus<\/i> \u0e0b\u0e36\u0e48\u0e07\u0e40\u0e1b\u0e47\u0e19\u0e40\u0e0a\u0e37\u0e49\u0e2d\u0e23\u0e32\u0e01\u0e48\u0e2d\u0e42\u0e23\u0e04\u0e23\u0e32\u0e01\u0e02\u0e32\u0e27\u0e43\u0e19\u0e15\u0e49\u0e19\u0e22\u0e32\u0e07\u0e1e\u0e32\u0e23\u0e32 \u0e16\u0e37\u0e2d\u0e40\u0e1b\u0e47\u0e19\u0e20\u0e31\u0e22\u0e04\u0e38\u0e01\u0e04\u0e32\u0e21\u0e2a\u0e33\u0e04\u0e31\u0e0d\u0e15\u0e48\u0e2d\u0e01\u0e32\u0e23\u0e1c\u0e25\u0e34\u0e15\u0e22\u0e32\u0e07\u0e18\u0e23\u0e23\u0e21\u0e0a\u0e32\u0e15\u0e34\u0e17\u0e31\u0e48\u0e27\u0e42\u0e25\u0e01 \u0e01\u0e32\u0e23\u0e17\u0e33\u0e04\u0e27\u0e32\u0e21\u0e40\u0e02\u0e49\u0e32\u0e43\u0e08\u0e01\u0e25\u0e44\u0e01\u0e01\u0e32\u0e23\u0e01\u0e48\u0e2d\u0e42\u0e23\u0e04\u0e02\u0e2d\u0e07\u0e40\u0e0a\u0e37\u0e49\u0e2d\u0e23\u0e32\u0e0a\u0e19\u0e34\u0e14\u0e19\u0e35\u0e49\u0e08\u0e36\u0e07\u0e40\u0e1b\u0e47\u0e19\u0e2a\u0e34\u0e48\u0e07\u0e2a\u0e33\u0e04\u0e31\u0e0d\u0e2a\u0e33\u0e2b\u0e23\u0e31\u0e1a\u0e01\u0e32\u0e23\u0e1e\u0e31\u0e12\u0e19\u0e32\u0e01\u0e25\u0e22\u0e38\u0e17\u0e18\u0e4c\u0e01\u0e32\u0e23\u0e08\u0e31\u0e14\u0e01\u0e32\u0e23\u0e42\u0e23\u0e04\u0e17\u0e35\u0e48\u0e21\u0e35\u0e1b\u0e23\u0e30\u0e2a\u0e34\u0e17\u0e18\u0e34\u0e20\u0e32\u0e1e \u0e40\u0e0a\u0e37\u0e49\u0e2d\u0e23\u0e32\u0e19\u0e35\u0e49\u0e1c\u0e25\u0e34\u0e15\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e17\u0e35\u0e48\u0e40\u0e23\u0e35\u0e22\u0e01\u0e27\u0e48\u0e32 &#8220;\u0e40\u0e2d\u0e1f\u0e40\u0e1f\u0e01\u0e40\u0e15\u0e2d\u0e23\u0e4c&#8221; \u0e0b\u0e36\u0e48\u0e07\u0e21\u0e35\u0e1a\u0e17\u0e1a\u0e32\u0e17\u0e2a\u0e33\u0e04\u0e31\u0e0d\u0e43\u0e19\u0e01\u0e32\u0e23\u0e1b\u0e23\u0e31\u0e1a\u0e40\u0e1b\u0e25\u0e35\u0e48\u0e22\u0e19\u0e01\u0e32\u0e23\u0e15\u0e2d\u0e1a\u0e2a\u0e19\u0e2d\u0e07\u0e20\u0e39\u0e21\u0e34\u0e04\u0e38\u0e49\u0e21\u0e01\u0e31\u0e19\u0e02\u0e2d\u0e07\u0e1e\u0e37\u0e0a\u0e41\u0e25\u0e30\u0e17\u0e33\u0e43\u0e2b\u0e49\u0e40\u0e1b\u0e47\u0e19\u0e42\u0e23\u0e04\u0e44\u0e14\u0e49\u0e07\u0e48\u0e32\u0e22\u0e02\u0e36\u0e49\u0e19 \u0e43\u0e19\u0e01\u0e32\u0e23\u0e28\u0e36\u0e01\u0e29\u0e32\u0e19\u0e35\u0e49 \u0e21\u0e35\u0e01\u0e32\u0e23\u0e43\u0e0a\u0e49\u0e27\u0e34\u0e18\u0e35\u0e0a\u0e35\u0e27\u0e2a\u0e32\u0e23\u0e2a\u0e19\u0e40\u0e17\u0e28\u0e40\u0e1e\u0e37\u0e48\u0e2d\u0e23\u0e30\u0e1a\u0e38\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e40\u0e2d\u0e1f\u0e40\u0e1f\u0e01\u0e40\u0e15\u0e2d\u0e23\u0e4c\u0e17\u0e35\u0e48\u0e40\u0e1b\u0e47\u0e19\u0e44\u0e1b\u0e44\u0e14\u0e49\u0e08\u0e33\u0e19\u0e27\u0e19 357 \u0e0a\u0e19\u0e34\u0e14\u0e08\u0e32\u0e01\u0e08\u0e35\u0e42\u0e19\u0e21\u0e02\u0e2d\u0e07 <i>R. microporus<\/i> \u0e1e\u0e23\u0e49\u0e2d\u0e21\u0e01\u0e31\u0e1a\u0e02\u0e49\u0e2d\u0e21\u0e39\u0e25 RNA-seq \u0e02\u0e2d\u0e07\u0e40\u0e0a\u0e37\u0e49\u0e2d\u0e23\u0e32\u0e17\u0e35\u0e48\u0e41\u0e2a\u0e14\u0e07\u0e01\u0e32\u0e23\u0e15\u0e2d\u0e1a\u0e2a\u0e19\u0e2d\u0e07\u0e2b\u0e25\u0e31\u0e07\u0e08\u0e32\u0e01\u0e2a\u0e31\u0e21\u0e1c\u0e31\u0e2a\u0e01\u0e31\u0e1a\u0e19\u0e49\u0e33\u0e22\u0e32\u0e07\u0e1e\u0e32\u0e23\u0e32 \u0e01\u0e32\u0e23\u0e27\u0e34\u0e40\u0e04\u0e23\u0e32\u0e30\u0e2b\u0e4c\u0e2b\u0e19\u0e49\u0e32\u0e17\u0e35\u0e48\u0e02\u0e2d\u0e07\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e40\u0e2d\u0e1f\u0e40\u0e1f\u0e01\u0e40\u0e15\u0e2d\u0e23\u0e4c\u0e1e\u0e1a\u0e27\u0e48\u0e32\u0e21\u0e35\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e08\u0e33\u0e19\u0e27\u0e19\u0e21\u0e32\u0e01\u0e17\u0e35\u0e48\u0e40\u0e01\u0e35\u0e48\u0e22\u0e27\u0e02\u0e49\u0e2d\u0e07\u0e01\u0e31\u0e1a\u0e04\u0e27\u0e32\u0e21\u0e2a\u0e32\u0e21\u0e32\u0e23\u0e16\u0e43\u0e19\u0e01\u0e32\u0e23\u0e01\u0e48\u0e2d\u0e42\u0e23\u0e04\u0e02\u0e2d\u0e07\u0e40\u0e0a\u0e37\u0e49\u0e2d\u0e23\u0e32 \u0e42\u0e14\u0e22\u0e40\u0e09\u0e1e\u0e32\u0e30\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e44\u0e2e\u0e42\u0e14\u0e23\u0e42\u0e1f\u0e1a\u0e34\u0e19 (hydrophobin) \u0e41\u0e25\u0e30\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e44\u0e01\u0e25\u0e42\u0e04\u0e44\u0e0b\u0e14\u0e4c\u0e44\u0e2e\u0e42\u0e14\u0e23\u0e40\u0e25\u0e2a (glycoside hydrolase) \u0e19\u0e2d\u0e01\u0e08\u0e32\u0e01\u0e19\u0e35\u0e49\u0e22\u0e31\u0e07\u0e21\u0e35\u0e01\u0e32\u0e23\u0e17\u0e33\u0e19\u0e32\u0e22\u0e42\u0e04\u0e23\u0e07\u0e2a\u0e23\u0e49\u0e32\u0e07\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e02\u0e2d\u0e07\u0e40\u0e2d\u0e1f\u0e40\u0e1f\u0e01\u0e40\u0e15\u0e2d\u0e23\u0e4c\u0e1a\u0e32\u0e07\u0e0a\u0e19\u0e34\u0e14\u0e17\u0e35\u0e48\u0e41\u0e2a\u0e14\u0e07\u0e2d\u0e2d\u0e01\u0e2a\u0e39\u0e07 \u0e1e\u0e1a\u0e27\u0e48\u0e32\u0e21\u0e35\u0e04\u0e27\u0e32\u0e21\u0e04\u0e25\u0e49\u0e32\u0e22\u0e04\u0e25\u0e36\u0e07\u0e01\u0e31\u0e1a\u0e42\u0e1b\u0e23\u0e15\u0e35\u0e19\u0e2d\u0e32\u0e21\u0e34\u0e42\u0e19\u0e40\u0e1b\u0e1b\u0e15\u0e34\u0e40\u0e14\u0e2a (aminopeptidase) \u0e22\u0e39\u0e1a\u0e34\u0e04\u0e27\u0e34\u0e15\u0e34\u0e19\u0e44\u0e25\u0e40\u0e01\u0e2a (ubiquitin ligase) \u0e2a\u0e40\u0e1f\u0e2d\u0e23\u0e39\u0e25\u0e34\u0e19 (spherulin) \u0e41\u0e25\u0e30\u0e42\u0e18\u0e21\u0e32\u0e15\u0e34\u0e19 (thaumatin) \u0e07\u0e32\u0e19\u0e27\u0e34\u0e08\u0e31\u0e22\u0e19\u0e35\u0e49\u0e0a\u0e48\u0e27\u0e22\u0e40\u0e1e\u0e34\u0e48\u0e21\u0e04\u0e27\u0e32\u0e21\u0e40\u0e02\u0e49\u0e32\u0e43\u0e08\u0e43\u0e19\u0e01\u0e25\u0e44\u0e01\u0e01\u0e32\u0e23\u0e01\u0e48\u0e2d\u0e42\u0e23\u0e04\u0e02\u0e2d\u0e07 <i>R. microporus<\/i> \u0e41\u0e25\u0e30\u0e19\u0e33\u0e40\u0e2a\u0e19\u0e2d\u0e41\u0e19\u0e27\u0e17\u0e32\u0e07\u0e43\u0e2b\u0e21\u0e48\u0e43\u0e19\u0e01\u0e32\u0e23\u0e1e\u0e31\u0e12\u0e19\u0e32\u0e27\u0e34\u0e18\u0e35\u0e04\u0e27\u0e1a\u0e04\u0e38\u0e21\u0e42\u0e23\u0e04\u0e23\u0e32\u0e01\u0e02\u0e32\u0e27\u0e43\u0e19\u0e2a\u0e27\u0e19\u0e22\u0e32\u0e07\u0e1e\u0e32\u0e23\u0e32<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Frontiers in Microbiology 15 (2024): 1439454 https:\/\/doi.org\/10.3389\/fmicb.2024.1439454 Rigidoporus microporus, the fungus that causes white root rot disease in rubber trees, is a major threat to natural rubber production worldwide. Understanding how this fungus causes disease is important for developing better ways to manage it. The fungus releases effector proteins, which help it weaken the rubber [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":13344,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[77,73,13],"tags":[],"class_list":["post-13343","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-th","category-publication-th","category-uncategorized-th"],"_links":{"self":[{"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/posts\/13343","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13343"}],"version-history":[{"count":2,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/posts\/13343\/revisions"}],"predecessor-version":[{"id":13346,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/posts\/13343\/revisions\/13346"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=\/wp\/v2\/media\/13344"}],"wp:attachment":[{"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13343"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13343"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/plantscience.sc.mahidol.ac.th\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13343"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}